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Immobilization of Mucor javanicus lipase by entrapping in alginate-silica hybrid gel beads with simultaneous cross-linking with glutaraldehyde
Authors:Jin Chuan Wu  Yew Kong Wong  Kiat Wei Chang  Chor Yong Tay  Yvonne Chow  Mmr Talukder
Institution:1. Institute of Chemical and Engineering Sciences, 1 Pesek Road, Jurong Island, Singapore, 627833wu_jinchuan@ices.a-star.edu.sg;3. Institute of Chemical and Engineering Sciences, 1 Pesek Road, Jurong Island, Singapore, 627833
Abstract:Mucor javanicus lipase was entrapped in alginate-silica hybrid gel beads with or without simultaneous cross-linking with glutaraldehyde. The activity and recovery of activity on immobilization of the enzyme entrapped in the hybrid beads were 1.4 and 1.7 times higher than those of the enzyme entrapped in the simple alginate beads. Entrapment with simultaneous cross-linking in the hybrid beads further improved the enzyme activity (1.6 times) and activity recovery (1.7 times) compared to those of the enzyme entrapped in the hybrid beads without simultaneous cross-linking. The leakage of the enzyme entrapped in the hybrid beads with simultaneous cross-linking was only 50% that of the enzyme entrapped in the simple alginate beads.
Keywords:Alginate  cross-linking  entrapment  hybrid gel  lipase  Mucor javanicus  silica
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