Isomaltose formation by free and immobilized dextransucrase |
| |
| Authors: | Sonja Berensmeier Hans-Joachim Jördening Klaus Buchholz |
| |
| Affiliation: | 1. Institute for Technical Chemistry, Forschungszentrum Karlsruhe, P.O. Box 3640, D-76021, Karlsruhe, GermanySonja.berensmeier@itc-wgt.fzk.de;3. Department for Carbohydrates, Technical University Braunschweig, Langer Kamp 5, D-38106, Braunschweig, Germany |
| |
| Abstract: | The acceptor reaction of dextransucrase from Leuconostoc mesenteroides NRRL-B512F with glucose as acceptor is of technical interest for isomaltooligosaccharide (IMOs) synthesis. Different experimental conditions were investigated for free and immobilized enzyme. The data for oligosaccharide formation up to a degree of polymerization 4 were correlated with a model developed earlier, and optimal reaction conditions for immobilized dextransucrase design and application were identified for later continuous application. Furthermore, stability was investigated for free and immobilized enzyme including stabilization by sugars. |
| |
| Keywords: | alginate dextransucrase fluidized bed immobilization isomaltooligosaccharide |
|
|
