Continuous production of 7-Aminocephalosporanic acid by immobilized cells of Pseudomonas diminuta |
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| Authors: | Dr V. K. Nigam S. Kundu P. Ghosh |
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| Affiliation: | 1. Birla Institute of Scientific Research, Statue Circle, Jaipur, Rajasthannigam_vinod@yahoo.com;3. School of Biochemical Engineering, Institute of Technology, Banaras Hindu University, Varanasi, India;4. Birla Institute of Scientific Research, Statue Circle, Jaipur, Rajasthan |
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| Abstract: | The continuous production of 7-ACA with immobilized whole cells of P. diminuta was carried out in a tubular glass reactor at optimal conditions. The biocatalyst was prepared by gel entrapment using chitosan, geletin and agar as immobilizing agents. The micro-organism was characterized in terms of cell-to-carrier ratios, diffusional properties, as well as storage and operational stability, etc., towards the production of 7-ACA. It was found that a cell to carrier ratio of 0.45 and a flow rate of 120 mL h?1, respectively, were most suitable for the conversion of CPC to 7-ACA. Operational stability was highest with chitosan, with a half life of 2106 h followed by gelatin, then agar as immobilizing supports. |
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| Keywords: | 7-ACA CPC acylase GL-7-ACA acylase immobilization packed bed bioreactor continuous production |
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