Stabilization of immobilized cathepsin L in non-aqueous medium |
| |
Authors: | Swati Sharma Ashwani Mittal Rita Sharma V.K. Gupta |
| |
Affiliation: | Department of Biochemistry, Kurukshetra University, Kurukshetra, Haryana, India |
| |
Abstract: | A lysosomal cysteine protease cathepsin L (3.4.22.15) purified from goat brain has been immobilized in calcium alginate beads in the presence of BSA through entrapment. Most favorable conditions for the entrapment were standardized as 3.0%(w/v) alginate and 1.5%(w/v) calcium chloride. Comparing the properties of free and immobilized enzyme using Z-Phe-Arg-4mβNA as chromogenic substrate, it was found that the immobilized enzyme could retain~70% of the original activity after five successive batch reactions. Vis-à-vis the free enzyme, immobilization conferred high stability to the enzyme both in the acidic and alkaline range, the enzyme lost no activity up to 60°C (Temperature stability for free enzyme is only up to 50°C). The pH optima for the enzyme shifted from 6.2 to 6.6 on entrapment. The increase in activity and stability of the enzyme in immobilized form even in the presence of high concentration of DMSO and ethanol is surprising and may make it useful for catalyzing organic reactions like trans-esterification and trans-amidation. |
| |
Keywords: | alginate DMSO ethanol cathepsin L cysteine protease |
|
|