Kinetics of Pyruvate Decarboxylase Deactivation by Benzaldehyde |
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Authors: | Noppol Leksawasdi Michael Breuer Bernhard Hauer Peter L Rogers |
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Institution: | 1. School of Biotechnology and Biomolecular Sciences, University of New South Wales, NSW 2052, Sydney;2. BASF-AG, Fine Chemicals and Biocatalysis Research, 67056 Ludwigshafen |
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Abstract: | Based on experimental data, a kinetic model for the deactivation of partially purified pyruvate decarboxylase (PDC) by benzaldehyde (0–200 mM) in MOPS buffer (2.5 M) has been developed. An initial lag period prior to deactivation was found to occur. With first order dependencies of PDC deactivation on exposure time and on benzaldehyde concentration, a reaction time deactivation constant of 2.64×10?3 h?1 and a benzaldehyde deactivation coefficient of 1.98×10?4 mM?1 h?1 were determined for benzaldehyde concentrations up to 200 mM. The PDC deactivation kinetic equations established in this study are an essential component in an overall model being developed to describe the enzymatic biotransformation of benzaldehyde and pyruvate to produce the pharmaceutical intermediate (R)-phenylacetylcarbinol (R-PAC). |
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Keywords: | (R)-phenylacetylcarbinol Candida utilis pyruvate decarboxylase Enzyme deactivation by benzaldehyde Mathematical modelling |
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