An efficient biotransformation of dialkyl esters of 2-oxoglutaric acid by Rhodotorula minuta whole cells |
| |
| Authors: | Eduardo M. Rustoy Patricia Cerrutti Miguel A. Galvagno |
| |
| Affiliation: | 1. Departamento de Química Orgánica y UMYMFOR, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellón 2, Piso 3, Ciudad Universitaria, C1428EGA, Buenos Aires, Argentina;2. Departamento de Ingeniería Química, Facultad de Ingeniería, Universidad de Buenos Aires, Pabellón de Industrias, Piso 1, Ciudad Universitaria, C1428EGA, Buenos Aires, Argentina;3. IIB-Intech-CONICET-UNSAM, Colectora Avenida General Paz 5445 Edificio 24 y 19, C1650, San Martín, Buenos Aires, Argentina |
| |
| Abstract: | Whole cells of the yeast Rhodotorula minuta were used in the biotransformation of dialkyl esters of 2-oxoglutaric acid. Almost 100% of conversion with 97–98% of enantiomeric excess of the (S) form of 2-hydroxydiesters was obtained through an enantioselective reduction of dimethyl and diethyl 2-oxoglutarate. When longer alkoxy chain 2-oxoglutarates were used as substrates, the corresponding 4-hydroxybutyric esters were obtained, suggesting a combination process including hydrolysis, decarboxylation and reduction. The cells showed a remarkable high productivity: high conversion and enantiomeric excess were obtained at 2 g wet weight mmol?1 substrate. |
| |
| Keywords: | Biotransformation dialkyl 2-oxoglutaric acid esters Rhodotorula minuta |
|
|
