Study of the Stability Of Vaccinium myrtillus Peroxidase in Reverse Micellar Systems |
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| Authors: | AJE Pedro PS Fevereiro ML Serralheiro MR Aires-Barros |
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| Institution: | 1. Centro de Engenharia Biológica e Química, Instituto Superior Técnico, Av. Rovisco Pais 1049-001 Lisboa, Portugal;2. Instituto de Tecnologia e Química Biológica (ITQB), Universidade Nova de Lisboa, Laboratório de Biotecnologia de Células Vegetais, Apartado 127, P-2780 Oeiras, Portugal;3. Departamento de Biologia Vegetal, Faculdade de Ciências, Universidade de Lisboa, Lisboa, Portugal;4. Centro de Ciências Moleculares e Materiais da Faculdade de Ciências da Universidade de Lisboa, Lisboa, Portugal |
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| Abstract: | The stability of a cationic peroxidase isolated and purified from a cell suspension of Vaccinium myrtillus, microencapsulated in reverse micelles of sodium dioctylsulfosuccinate (AOT) was evaluated. By using a central composite design (CCD), some relevant parameters for the enzymatic activity, such as surfactant and water concentration, pH and buffer molarity, were analysed. The response surface curves showed that 50 mM AOT, 500 mM water, 80 mM buffer and pH 7.6 were the best conditions for enzyme stability. The effect of carbohydrates and polyols on enzyme stability was also evaluated. At 20 mM, carbohydrates like arabinose, and trehalose increased the enzymatic stability by a factor of 4.4 and 2.3, respectively, but melezitose had no effect. From the three polyols tested, inositol and sorbitol increased the peroxidase stability by a factor of 3.8 and 1.8, respectively, while mannitol had no effect. |
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| Keywords: | Cationic Peroxidase Vaccinium Myrtillus Reverse Micelles Stability Additive |
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