Effects and statistical optimization of fermentation conditions on growth and poly (vinyl alcohol)-degrading enzyme production of Streptomyces venezuelae GY1 |
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| Authors: | Ying Zhang Guocheng Du Xuerong Fan |
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| Affiliation: | 1. Key Laboratory of Eco-Textiles (Jiangnan University), Ministry of Education, School of Textiles and Clothing, Jiangsu, China;2. The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Jiangsu, China |
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| Abstract: | The effects of environmental conditions, including temperature, pH and dissolved oxygen, on growth and production of polyvinyl alcohol (PVA)-degrading enzymes of the newly-isolated strain Streptomyces venezuelae GY1 were investigated. The medium composition for strain GY1 was studied first by single factorial design and then optimized using a central composite design. PVA with high saponification is better for growth of, and PVA-degrading enzyme production by S. venezuelae GY1 compared with PVA with low saponification, in contrast with the characteristics of other bacteria producing PVA-degrading enzymes. The optimal temperature and initial pH for production of PVA-degrading enzyme by strain GY1 was 30°C and 7.0, respectively. The optimal medium composition for PVA-degrading enzyme production is: 1.01 g L?1 of PVA1799, 0.307 g L?1 of NaNO3 and 0.512 g L?1 of MgSO4?7H2O. |
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| Keywords: | PVA-degrading enzyme central composition design optimization |
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