Stereoselective hydrolysis of racemic ethyl 4-chloro-3-hydroxybutyrate by a lipase |
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| Authors: | Sunho Chung Yunesahng Hwang |
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| Affiliation: | 1. Department of Bioprocess Engineering, Institute of Bio-Chem, Enzytech, Ltd., Daejoncafe1127@hanmail.net;3. Korean Minjok Leadership Academy, Gangwon-do, Korea |
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| Abstract: | The stereoselective hydrolysis of racemic ethyl 4-chloro-3-hydroxybutyrate (ECHB) was performed by using Novozym 435 lipase in an aqueous phase. It was found that racemic ECHB was hydrolysed to (R)-ECHB and (S)-3-hydroxy-gamma-butyrolactone (HGBL) via (S)-4-chloro-3-hydroxybutyric acid. From this result, (R)-ECHB (99%ee) was produced in a good yield on a preparative scale. |
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| Keywords: | Ethyl 4-chloro-3-hydroxybutyrate hydrolysis 3-hydroxy-gamma-butyrolactone Novozym 435 stereoselectivity |
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