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Performance of an immobilized fuculose-1-phosphate aldolase for stereoselective synthesis
Authors:Trinitat Suau  Gregorio Álvaro  Josep López-Santín
Institution:Departament d'Enginyeria Química, Escola Tècnica Superior d'Enginyeria, Unitat de Biocatàlisi Aplicada associada al IIQA (CSIC), Universitat Autònoma de Barcelona, Bellaterra, Spain
Abstract:A derivative of fuculose-1-phosphate aldolase, immobilized with high loading on glyoxal–agarose gels, has been characterized and evaluated as a biocatalyst for an aldol addition reaction. The reaction of the solid biocatalyst was diffusion-controlled for conversion of its natural substrate. Nevertheless, when catalyzing the synthesis of a biologically active aminopolyol, the lower reaction rate with non-natural substrates led to a process controlled by the intrinsic enzyme kinetics. The resulting biocatalyst has high synthetic specific activity and has been successfully used in batch synthesis reactions with high conversion. In addition, the immobilized aldolase has been employed in fed-batch synthesis, increasing the selectivity of the reaction and obtaining high conversion (88%).
Keywords:Fuculose-1-phosphate aldolase  immobilized enzymes  aldol addition  diffusional limitations  specific activity
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