An improved multi-element measurement of mineral absorption in the piglet utilizing the fecal monitoring technique |
| |
Authors: | Rosalind S Gibson Ian L Gibson Colin E Webber Stephanie A Atkinson |
| |
Institution: | (1) Applied Human Nutrition, Department of Family Studies, University of Guelph, Guelph, Ontario, Canada;(2) Department of Earth Sciences, University of Waterloo, Waterloo, Ontario, Canada;(3) Department of Nuclear Medicine, McMaster University, L8N 3Z5 Hamilton, Ontario, Canada;(4) Department of Pediatrics, McMaster University, L8N 3Z5 Hamilton, Ontario, Canada |
| |
Abstract: | The fecal monitoring technique for measuring the absorption of Zn, Mn, Se, and Fe was studied in eight male piglets (mean±SEM
birthweight (bw)=1695±50 g) using high resolution gamma spectrometry. Four d old piglets were fed a complete liquid milk diet
for five d prior to the orogastric administration of an isotope dose (75Se,54Mn,59Fe, and65Zn) equilibrated with the liquid milk diet.51CrCl3 was used as a fecal marker but was found to be partially absorbed. Stool samples were collected daily for 15 d, counted,
and then the daily fecal excretion was calculated. Results indicate that endogenous excretion for each of the isotopes was
not constant but decreased exponentially with time. The pattern of endogenous excretion varied between elements. An improved
method for calculating the endogenous excretion was therefore developed. This method is based on the pattern of endogenous
excretion in three-four d old male piglets (mean±SEM bw=2060±75 g) injected intravenously with the same isotopes and on the
level of endogenous excretion in orally fed animals in the postabsorptive phase of excretion. These findings have important
implications for the estimation of endogenous excretion in future fecal monitoring absorption studies in order to minimize
underestimation of true absorption. |
| |
Keywords: | Mineral nutrition true absorption endogenous excretion piglet fecal monitoring |
本文献已被 SpringerLink 等数据库收录! |
|