Structure of carbohydrate chains of a high-molecular-weight pulmonary glycoprotein |
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Authors: | Saura C Sahu William S Lynn |
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Institution: | Departments of Biochemistry and Medicine, Duke University Medical Center, Durham, North Carolina 27710 U.S.A. |
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Abstract: | A human, alveolar glycoprotein having an apparent mol. wt. of 250 000 gave two major glycopeptide fractions (I and II) by Pronase digestion, followed by gel filtration, DEAE-cellulose column chromatography, paper chromatography, and paper electrophoresis. Glycopeptide I contained d-galactose, d-mannose, 2-acetamido-2-deoxy-d-glucose, and N-acetylneuraminic acid in the molar ratio of 2:3:4:1, whereas these sugars were present in Glycopeptide II in the molar ratio of 2:3:4:2.l-Fucose was present only in Glycopeptide II at a concentration of one l-fucose per three d-mannose residues. In both glycopeptides, 2-acetamido-2-deoxy-d-glucose was linked to an asparagine residue of the peptide chain. Based on the results of alkaline borohydride treatment, periodate oxidation, methlylation analysis, and sequential glycosidase degradation of the glycopeptides, tentative structures are proposed for both glycopeptides. |
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Keywords: | To whom all Correspondence should be directed at the present address: C P S C Health Sciences Laboratories 200 C Street S W Washington DC 20204 (U S A ) |
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