Effects of source of macronutrients and plant growth regulator concentrations on shoot proliferation of Cornus florida |
| |
Authors: | Veronique Declerck Schuyler S Korban |
| |
Institution: | (1) Department of Horticulture, University of Illinois, 1201 W. Gregory, 310 PABL, 61801 Urbana, IL, USA;(2) Present address: I.S.A.B., 32 Boulevard du Port, 95094 Cergey-Pontoise Cedex, France |
| |
Abstract: | Nodal stem segments of flowering dogwood (Cornus florida L.) were cultured on media containing seven different sources of macronutrients including full- and half-strength Murashige & Skoog (MS) macrosalts, N6, Anderson's (AND), Quoirin & Lepoivre's (LP), Nitsch & Nitsch (NIT), and Woody Plant Medium (WPM). All media contained MS micronutrients, Staba vitamins, 20 g l-1 sucrose, and 6.5 g l-1 Difco Bacto-agar, and were supplemented with 2.2 M 6-benzyladenine (BA) and 0.49 M indole-3-butyric acid (IBA). Following three subcultures, the best shoot proliferation was supported on media containing WPM macronutrients. To optimize the proliferation rate, shoots were cultured on WPM macronutrients supplemented with eight combinations of BA and IBA, and 3.3 M BA without IBA was determined to be the best.Abbreviations BA
6-benzyladenine
- IBA
indole-3-butyric acid |
| |
Keywords: | cytokinins auxins flowering dogwood tissue culture |
本文献已被 SpringerLink 等数据库收录! |
|