基于ITS和trnL-F以及rps4基因片段的 粗疣藓属分类学地位研究

选择粗疣藓属及相关属样本作为内群,油藓属样本作为外群,选取核基因ITS片段以及叶绿体基因trnL-F和rps4片段进行PCR扩增、测序,运用贝叶斯推论(BI)、最大似然法(ML)和最大简约法(MP)构建分子系统发育树,以明确粗疣藓属的分类学地位。结果表明:(1)用于分子系统分析的基因序列共有1 744个位点,其中747个位点属于核基因片段,997个位点属于叶绿体基因片段。(2)3种系统发育树拓扑结构基本一致,都显示粗疣藓属与毛锦藓科成员聚成一支,支持粗疣藓属应隶属于毛锦藓科的观点。(3)小粗疣藓和粗疣藓的种间界限模糊。(4)小鼠尾藓属的3个种与棉藓属成员聚成一支,支持将其归入棉藓科的观点。

Taxonomic Status of Fauriella Besch.Inferred from ITS,trnL-F and rps4 Gene Regions

To clarify the obscured taxonomic status of Fauriella Besch.,we carried out phylogenetic analyses inferred from cp trnL-F,rps4 and nr ITS,with increased samples of Fauriella and related genera,and Hookeria Sm.selected as outgroup.The phylogenetic trees were conducted by Bayesian analyses,ML analyses,and MP analyses.The results were as follows:(1)The final alignment used for phylogenetic analyses contained 1 744 positions,including 747 nuclear region positions and 997 plastid positions;(2)There was no significant topological conflict among the consensus trees,which showed that Fauriella clustered with the members of Pylaisiadelphaceae and indicated that Fauriella should be put into Pylaisiadelphaceae;(3)The relationship between Fauriella tenerrima Broth.and Fauriella tenuis (Mitt.) Cardot is obscured;(4)3 species of Myurella Bruch & Schimp.are clustered with the members of Plagiothecium Bruch & Schimp.,which is congruent with the point that Myurella should be transferred to Plagiotheciaceae.