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Aeration strategy: a need for very high ethanol performance in<Emphasis Type="Italic"> Saccharomyces cerevisiae</Emphasis> fed-batch process
Authors:S?Alfenore  X?Cameleyre  L?Benbadis  C?Bideaux  J-L?Uribelarrea  G?Goma  C?Molina-Jouve  Email author" target="_blank">S?E?GuillouetEmail author
Institution:(1) Biotechnology and Bioprocess Laboratory, UMR-CNRS 5504, UMR-INRA 792, Institut National des Sciences Appliquées, Département de Génie Biochimique et Alimentaire, 135 Avenue de Rangueil, 31077 Toulouse Cedex, France;(2) CRT CRITT Bioindustries, Institut National des Sciences Appliquées, Département de Génie Biochimique et Alimentaire, 135 Avenue de Rangueil, 31077 Toulouse Cedex, France
Abstract:In order to identify an optimal aeration strategy for intensifying bio-fuel ethanol production in fermentation processes where growth and production have to be managed simultaneously, we quantified the effect of aeration conditions—oxygen limited vs non limited culture (micro-aerobic vs aerobic culture)—on the dynamic behaviour of Saccharomyces cerevisiae cultivated in very high ethanol performance fed-batch cultures. Fermentation parameters and kinetics were established within a range of ethanol concentrations (up to 147 g l–1), which very few studies have addressed. Higher ethanol titres (147 vs 131 g l–1 in 45 h) and average productivity (3.3 vs 2.6 g l–1 h–1) were obtained in cultures without oxygen limitation. Compared to micro-aerobic culture, full aeration led to a 23% increase in the viable cell mass as a result of the concomitant increase in growth rate and yield, with lower ethanol inhibition. The second beneficial effect of aeration was better management of by-product production, with production of glycerol, the main by-product, being strongly reduced from 12 to 4 g l–1. We demonstrate that aeration strategy is as much a determining factor as vitamin feeding (Alfenore et al. 2002) in very high ethanol performance (147 g l–1 in 45 h) in order to achieve a highly competitive dynamic process.
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