Isolation and characterization of a novel equol-producing bacterium from human feces |
| |
Authors: | Yokoyama Shin-ichiro Suzuki Tohru |
| |
Institution: | Department of Applied Microbiology, Gifu Prefectural Research Institute for Bioengineering, Gifu, Japan. yokoyama@bio.rd.pref.gifu.jp |
| |
Abstract: | An equol-producing bacterium was newly isolated from the feces of healthy humans and its morphological and biochemical properties were characterized. The cells were obligate anaerobes. They were non-sporulating, non-motile, gram-positive bacilliform bacteria with a pleomorphic morphology. The strain was catalase-positive, and oxidase-, urease-, and indole-negative. The only other sugar utilized by the strain was glycerin. The strain also degraded gelatin, but not esculin. It was most closely related to Eggerthella hongkongensis HKU10, with 93.3% 16S rDNA nucleotide sequence homology. Based on these features, the isolate was identified as a novel species of the genus Eggerthella. It was named Eggerthella sp. YY7918. Strain YY7918 converted substrates daidzein and dihydrodaidzein into S-equol, but did not convert daidzin, glysitein, genistein, or formononetin into it. An antimicrobial susceptibility assay indicated that strain YY7918 was susceptible to aminoglycoside-, tetracycline-, and new quinolone-antibiotics. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|