Physicochemical properties of diheme cytochrome c4 of unknown function from Vibrio parahaemolyticus strain RIMD2210633 |
| |
| Authors: | Akazaki Hideharu Futami Yoshio Shibayama Naoya Shirasaki Ikuko Nakade Harumi Chida Hirotaka Hakamata Wataru Park Sam-Yong Nishio Toshiyuki Oku Tadatake |
| |
| Institution: | Bio-Organic Chemistry Laboratory, Graduate School of Bioresource Sciences, Nihon University, Kanagawa, Japan. |
| |
| Abstract: | To characterize a diheme cytochrome c4 of unknown functional of the Vibrio genus for the first time, the Vibrio parahaemolyticus cytochrome c4 was overexpressed in Escherichia coli periplasm using the endogenous signal sequence. The physicochemical properties of the purified recombinant protein, viz., molecular mass, UV/Vis, and CD spectra, and the redox potentials of the N- and C-terminal domain hemes were determined. |
| |
| Keywords: | |
| 本文献已被 PubMed 等数据库收录！ |
|
