Thin frozen film method for visualization of storage proteins in mature rice grains |
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Authors: | Saito Yuhi Nakatsuka Nobuaki Shigemitsu Takanari Tanaka Kunisuke Morita Shigeto Satoh Shigeru Masumura Takehiro |
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Affiliation: | Laboratory of Genetic Engineering, Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, Kyoto, Japan. |
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Abstract: | There are technical difficulties in obtaining intact sections of cereal grains in which mature cells and their subcellular structures are well preserved. Here we describe a simple method for sectioning hard mature rice grains. It makes possible accurate localization of storage proteins in high-quality histological sections of rice endosperm. |
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