Affinity chromatography of mustard β-amylase on starch columns |
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Authors: | K. Subbramaiah R. Sharma |
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Affiliation: | School of Life Sciences, University of Hyderabad, Hyderabad-500 134, India |
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Abstract: | An affinity chromatography method for purification of β-amylase from cytoledons of whit mustard seedlings (Sinapsi alba L.) is described. β-Amylase is bound to starch column, while other contaminating proteins are eluted with the binding buffer. The bound β-amylase is eluted by including dextrin (1%, w/v) in binding buffer. This method yielded a homogenous preparation of β-amylase enzyme, which migrated as a single polypetide band in SDS electrophoresis. |
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Keywords: | affinity chromatography β-amylase starch column |
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