Inhibitory effects of tunicamycin on cell-cell adhesion and cell reaggregation of the cellular slime mold,Polysphondylium pallidum |
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Authors: | Nobuyuki Nakata Hiroshi Ochiai |
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Institution: | (1) Division of Biological Sciences, Graduate School of Science, Hokkaido University, Kita-ku, 060 Sapporo, Hokkaido, Japan |
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Abstract: | To assess the role in cell-cell adhesion of gp64, a putative cell-cell adhesion molecule ofPolysphondylium pallidum, we treated the cells with tunicamycin (TM), a known inhibitor of the synthesis of the N-linked oligosaccharide precursor,
and examined TM's effect on cell-cell adhesion. The vegetative growth ofPolysphondylium cells was inhibited with TM in a dose-dependent manner. When cells were treated with TM (2.0 μg/ml) during only the first
4 hr of starvation and further starved for 8 hr without TM, the cells dissociated considerably, although even the growth phase
cells ofPolysphondylium normally show EDTA-resistant (Ca2+-independent) cell adhesions. In parallel with the above effects, the amounts of intact gp64 decreased considerably in time
with the lengths of incubation (0 hr>4 hr >8 hr). When TM-treated cells were washed free of TM, and shaken for a further 12
hr, the cells began to aggregate again, accompanied by an increase of gp64. In conclusion, TM affected cell-cell adhesion
ofPolysphondylium cells, but we were not able to distinguish whether the inhibition of cell aggregation was due to defects in glycosylation
on glycoproteins and/or due to reduced levels of glycoproteins themselves. |
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Keywords: | Cell-cell adhesion Cellular slime mold Glycoprotein Polysphondylium pallidum Tunicamycin |
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