Taenia taeniaeformis in mice: passive transfer of protection with sera from infected or vaccinated mice and analysis of serum antibodies to oncospheral antigens期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

Taenia taeniaeformis in mice: passive transfer of protection with sera from infected or vaccinated mice and analysis of serum antibodies to oncospheral antigens

Authors:	M W Lightowlers M D Rickard G F Mitchell

Affiliation:	1. University of Melbourne, Veterinary Clinical Centre, Princes Highway, Werribee, Victoria, 3030, Australia;1. Immunoparasitology Unit, Walter and Eliza Hall Institute, Melbourne, Victoria, 3050, Australia;1. Haskins Laboratories, and Department of Chemistry and Physical Sciences, Pace University, New York, USA;2. Cummings School of Veterinary Medicine, Tufts University, N. Grafton, MA, USA;3. Analytical Imaging Facility, Albert Einstein College of Medicine, Bronx, NY, USA;4. Department of Pathology, Albert Einstein College of Medicine, Bronx, NY, USA;5. Department of Medicine, Albert Einstein College of Medicine, Bronx, NY, USA;1. Department of Immunology, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran;2. Clinical Tuberculosis and Epidemiology Research Center, National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti University of Medical Sciences, Tehran, Iran;3. Airways Disease Section, National Heart and Lung Institute, Imperial College London, London, UK;4. Infectious Diseases and Tropical Medicine Research Center, Shahid Beheshti, University of Medical Sciences,Tehran, Iran;5. Department of Pathology and Laboratory Medicine, University of California, Los Angeles (UCLA), USA;6. Division of Pharmacology, Utrecht Institute for Pharmaceutical Sciences, Faculty of Sciences, Utrecht University, Utrecht, The Netherlands;7. Department of Immunology, Nutricia Research, Utrecht, the Netherlands;8. Chronic Respiratory Diseases Research Center and National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti University of Medical Sciences, Tehran, Iran;9. Mycobacteriology Research Center, National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti University of Medical Sciences, Tehran, Iran;1. Institute of Parasitology, Justus Liebig University Giessen, Schubertstrasse 81, 35392, Giessen, Germany;2. Diamond V, Cedar Rapids, IA, 52404, USA;3. Unit of Biomathematics and Data Processing, Faculty of Veterinary Medicine, Justus Liebig University, Giessen, Germany;1. Department of Population Medicine, Ontario Veterinary College, University of Guelph, 50 Stone Road E Guelph, Ontario N1G 2W1, Canada;2. Centre for Veterinary Epidemiologic Research, Department of Health Management, Atlantic Veterinary College, University of Prince Edward Island, 550 University Avenue, Charlottetown, Prince Edward Island C1A 4P3, Canada;3. Department of Pathobiology, Ontario Veterinary College, University of Guelph, 50 Stone Road E Guelph, Ontario N1G 2W1, Canada;1. Departamento de Ciências Patológicas, Laboratório de Parasitologia, Universidade Estadual de Londrina, PR, Brazil;2. Departamento de Imunologia, Microbiologia e Parasitologia, Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, MG, Brazil;3. Ambulatório de Especialidades do Hospital Universitário de Londrina, PR, Brazil;4. Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo, SP, Brazil

Abstract:	Passive transfer of immunity to Taenia taeniaeformis infection was investigated using sera either from mice protected against infection by vaccination with oncosphere antigen or from mice 28 days after infection (28 DPI) with eggs. Transfer of 0.2 ml serum from mice vaccinated with either freeze-thaw-sonicated oncospheres (TtO FTS) or solubilized oncospheres (TtO DOC) failed to provide protection against infection with larvae in the recipient mice despite the fact that the serum donors were completely protected against the development of any viable larvae from a challenge infection with eggs. Some transfer of protection was achieved using larger volumes of sera (0.25, 0.5 ml) from vaccinated mice and by using sera collected from vaccinated mice 7 days after a challenge infection with eggs. The levels of protection provided with these sera were however always inferior to that achieved by passive transfer with sera collected from mice 28 DPI. Anti-oncospheral antibody levels in the sera used for passive transfer experiments were analysed using an Enzyme Linked Immunosorbent Asay (ELISA) and by Western blot immunoassay of oncosphere antigens separated in deoxycholate polyacrylamide gel electrophoresis (DOC PAGE). Differences in the levels of passive protection transferred with sera from 28 DPI mice and vaccinated mice did not correlate with anti-oncospheral antibody responses detected in ELISA. A correlation was evident between passive protection and detection of antibodies against an oncospheral antigen having a relative mobility of 0.62 in 10% acrylamide DOC PAGE. Gel cut-outs containing this antigen did not vaccinate mice against infection and loss of important conformational determinants of this antigen is suggested as a possible explanation for this. A simple association was not evident between vaccination-induced protection against infection and the presence of serum antibodies capable of passively transferring protection. The relevance of these observations for the rational selection of antibody probes for in vitro analysis of vaccine antigens is discussed.

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