Atrial natriuretic factor-induced cGMP accumulation in rat anterior pituitary cells in culture is not coupled to hormonal secretion |
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| Authors: | J Simard J F Hubert F Labrie E Isra?l-Assayag S Heisler |
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| Institution: | 1. Medical Research Council Group in Molecular Endocrinology, Le Centre hospitalier de l''Université Laval, Sainte-Foy, Québec, Canada;2. Unité de Biorégulation cellulaire, Le Centre hospitalier de l''Université Laval, Sainte-Foy, Québec, Canada;1. INRA, Nutrition et Neurobiologie Intégrée, UMR 1286, 33076 Bordeaux, France;2. Univ. Bordeaux, Nutrition et Neurobiologie Intégrée, UMR 1286, 33076 Bordeaux, France;3. PROTECT, INSERM, Université Paris Diderot, Sorbonne Paris Cité, F-75019 Paris, France;4. Centre National de la Recherche Scientifique, Institut de Neurosciences Cognitives et Intégratives d''Aquitaine, Uité Mixte de Recherche 5287, 33076 Bordeaux, France;5. Université de Bordeaux, Institut de Neurosciences Cognitives et Intégratives d''Aquitaine, 33076 Bordeaux, France;6. Centre des Sciences du Goût et de l''Alimentation, AgroSup Dijon, CNRS, INRA, Université Bourgogne Franche-Comté, Dijon, France;7. Centre for the Developing Brain, Department of Division of Imaging Sciences and Biomedical Engineering, King’s College London, King’s Health Partners, St. Thomas’ Hospital, London SE1 7EH, United Kingdom;1. Lamar University, Department of Civil and Environmental Engineering, PO BOX 10024, Beaumont, TX 77710, United States;2. Oak Ridge National Laboratory, National Transportation Research Center, 2360 Cherahala Boulevard, Knoxville, TN 37932, United States;1. National Reference Centre for Arboviruses, Department of Microbiology, Medical School, Aristotle University of Thessaloniki, Thessaloniki 54124, Greece;2. Hellenic Centre for Disease Control and Prevention (HCDCP), Athens 15123, Greece;3. Division of Infectious Diseases, Department of Internal Medicine, University Hospital of Patras, 26500 Patras, Greece;4. Laboratory of Biological Control of Pesticides, Benaki Phytopathological Institute, Athens 14561, Greece;5. Department of Microbiology, School of Medicine, University of Athens, 11527 Athens, Greece;6. Department of Hygiene and Epidemiology, Faculty of Medicine, University of Thessaly, Larissa 41222, Greece;1. Department of Chemical Engineering, University of Cape Town, Rondebosch, Cape Town 7700, South Africa;2. Department of Mining Engineering, College of Engineering, University of Liberia, Capitol Hill, P.O. Box 9020, Monrovia, Liberia;3. Economic Geology Research Institute (EGRI), School of Geosciences, University of the Witwatersrand, 1 Jan Smuts Avenue, Braamfontein 2000, Johannesburg, South Africa |
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| Abstract: | While atrial natriuretic factor (ANF) does not influence ACTH secretion, it was reported to have a marked stimulatory effect on the intracellular accumulation of cGMP in rat anterior pituitary cells in culture. Since many biological actions of ANF appear coupled to its excitatory action on target cell guanylate cyclase, the current study was designed to characterize the ANF-induced cGMP response in anterior pituitary with a view to determining whether the nucleotide plays a regulatory role in the secretory function of this gland. A 3 min exposure of cells in primary culture to 300 nM ANF (99-126) or 100 microM sodium nitroprusside (SNP), a stimulator of guanylate cyclase, causes maximal 10- and 3-fold elevations of cGMP levels, respectively. Following a progressive decrease, 6- and 2-fold increases over basal cGMP levels are still observed after 180 min of incubation with ANF (99-126) and SNP, respectively. The half-maximal stimulation of cGMP accumulation induced by a 10 min exposure to ANF (99-126), or rat atriopeptin II (ANF 103-125) is observed at 9 +/- 2 and 125 +/- 22 nM, respectively. ANF fragments (99-109) and (111-126), as well as human cardiodilatin (hANF 1-16), do not alter cGMP levels. Basal and ANF-induced cGMP levels are at least 10-fold higher in cell populations enriched in gonadotrophs compared to gonadotroph-impoverished preparations. A 3 h incubation of cells with ANF (0.1-1000 nM), however, fails to modify spontaneous or LHRH-induced LH secretion. Similarly, ANF does not alter spontaneous release of GH, TSH or PRL. The data suggest indirectly that gonadotrophs represent a principal site at which ANF acts to stimulate cGMP synthesis, but that the nucleotide is not a specific regulator of the LH secretory process; nor is it generally involved as a second messenger in the secretory function of any cell type of the anterior pituitary gland. |
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