Sex-hormone-binding globulin and albumin concentrations in human cerebrospinal fluid |
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Authors: | R S?derg?rd T B?ckstr?m |
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Affiliation: | 1. Vita-Salute San Raffaele University, Milan, Italy;2. Department of Clinical Neuroscience Mood Disorders Unit, IRCCS San Raffaele Scientific Institute, Milan, Italy;3. Department of Clinical Neuroscience, Sleep Disorders Centre, IRCCS San Raffaele Scientific Institute, Italy |
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Abstract: | Polyacrylamide gel electrophoresis of plasma and concentrated cerebrospinal fluid (CSF) preincubated with tritium labelled 5 alpha-dihydrotestosterone (DHT) showed identical migration of the radioactivity, indicating the presence of sex-hormone-binding globulin (SHBG) in human CSF. The concentrations of SHBG (measured as the binding capacity) and albumin were measured in concentrated CSF (12 women and 1 man) and samples of plasma of some patients (9 women). SHBG could not be detected in 6 of the CSF samples, and the mean value of the determinable samples was 42.3 +/- 13.4 pmol/l. The mean +/- SE of the SHBG concentration in plasma was 90.8 +/- 8.9 nmol/l and the mean albumin concentrations in CSF and plasma were 3.4 +/- 0.6 mumol/l and 670 +/- 107 mumol/l respectively. The distribution ratio for SHBG over the blood-CSF barrier was 10 times higher than for albumin. It was concluded that the SHBG-binding in the CSF is negligible but that the albumin-binding may contribute to the CSF concentrations of testosterone and estradiol, which are 10-25% above the plasma unbound concentrations. |
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