Levels of 4-hydroxystilbene-oxidizing isoperoxidases related to constitutive disease resistance in in vitro-cultured grapevine |
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Authors: | Antonio A Calderón José M Zapata María A Pedreño Romualdo Muñoz Alfonso Ros Barceló |
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Institution: | (1) Department of Plant Biology, University of Murcia, Campus of Espinardo, E-30071 Murcia, Spain |
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Abstract: | A zymographic screening of peroxidases (EC 1.11.1.7) capable of oxidizing 4-hydroxystilbene was carried out by means of the peroxidase-catalyzed oxidative coupling of 4-hydroxystilbene and 4-aminoantipyrine. This screening reveals that only a few isoperoxidases are active in oxidizing 4-hydroxystilbene to viniferin-type compounds in in vitro cultures of grapevine. Unlike total peroxidase activity measured with 4-methoxy--naphthol, the levels of total peroxidase activity measured using 4-hydroxystilbene are related to disease resistance against downy mildew in axillary bud cultures of Vitis vinifera and (Vitis vinifera x Vitis rupestris) x Vitis riparia. From this observation, and using the above zymographic assay, it was found that a 4-hydroxystilbene-oxidizing isoperoxidase was overexpressed in both leaves and stems of the hybrid in relation to the increase in disease resistance of this species. These results suggest that constitutive 4-hydroxystilbene-oxidizing isoperoxidases may participate through their role in viniferin synthesis in the constitutive resistance mechanism that grapevines show against downy mildew.Abbreviations 4-AAP
4-aminoantipyrine
- HRP
horseradish peroxidase
- 4-HS
hydroxystilbene
- HSPrx
4-hydroxystilbene-oxidizing peroxidase
- 4-MN
4-methoxy--naphthol |
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Keywords: | axillary bud culture cell culture peroxidase isoenzymes viniferin synthesis Vitis vinifera (Vitis vinifera x Vitis rupestris) x Vitis riparia |
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