粗柄鸡菌菌丝体培养和无性繁殖过程研究初报

鸡菌属菌物是一类具有商业前景的食物蕈菌。对Ｔｅｒｍｉｔｏｍｙｃｅｓａｌｂｕｍｉｎｏｓｕｓ（Ｂｅｒｋ）Ｈｅｉｍ进行了广泛研究，其它种类的研究仅见于Ｔ．ｆｕｌｇｉｎｏｓｕｓ（Ｂｅｒｋ．）Ｈｅｉｍ。笔者首次对Ｔ．ｒｏｂｕｓｔｕｓ（Ｂｅｅｌｉ）Ｈｅｉｍ粗柄鸡菌进行了纯培养研究，其菌丝体生长所需的碳源以麦芽糖为佳，葡萄糖、蔗糖次之；氮源以蛋白胨、酵母粉较好；天然材料的热水提取物，如松针、蚁巢圃、马铃薯、平菇出菇废料、蚕豆的混合物能促进菌丝生长；培养基在ｐＨ４．５左右最佳，可用０．０５％的柠檬酸调节。菌丝体纯培养很容易形成大量分生孢子和小白球菌，表明小白球菌是粗柄鸡菌菌丝体无性繁殖过程的一个阶段。

STUDY ON THE CULTURE OF MYCELIUM AND THE PROCESS OF ASEXUAL REPRODUCTION OF TERMITOMYCES ROBUSTUS

Termitomyces Heim(1941) is a kind of edible mushrooms with a good business prospects.T.albuminosus, has been widely studied, However, a little research in the others species has been done, but the species of T. fulginosus. For the first time, the auther has studied the culture of mycelium of T. robustus (Beeli) Heim . The results show that the optimum conditions for the growth of this mushroom are: maltose,glucose and sucrose as carbon source, peptone and yeast powder as nitrogen source. Hot water extract of nature materials, such as pine needle, fungus comb of termite nest, potato, the waste material of culture of oyster mushroom and broad bean,can promote its growth.The optimum pH value of the medium is about pH4.5, it can be adjusted with 0.05% citric acid Pure culture of the mycelium of this mushroom is easy to form a great amount of conidium and spherule.It suggested that the spherule is a stage of the asexual reproduction of T.robustus.