优美红游动菌类胡萝卜素的提取条件研究

研究了优美红游动菌(Rhodoplanes elegans)的生长以及类胡萝卜素的产生,通过比较4种广泛采用的细胞破碎方法,对优美红游动菌(Rhodoplanes elegans)类胡萝卜素的提取条件进行了研究,结果表明:类胡萝卜素的产生与菌种的生长曲线相符合,菌种活化后培养45h,类胡萝卜素的含量趋于稳定;取此时培养液,采用超声波法破碎细胞(破碎功率640W,时间10min),类胡萝卜素提取效果最好,初提液类胡萝卜素的提取率约为7.62mg·(g干菌体)^-1;酸热法对色素的破坏严重。这为进一步开发利用天然色素资源提供了一定的理论依据。

Study on the extraction of carotenoids in Rhodoplanes elegans

Four different cell broken methods were compared to find out the best extraction method of carotenoid pigment from Rhodoplanes elegans. The results showed that the output of carotenoid pigment was in accordance with the growth curve of the strain; the content of carotenoid pigment tended to be constant after culturing the activated strain for 45h; the best extraction method was that the cells obtained as above were broken by ultrasonic at 640W for 10 min. The extracting ratio of carotenoid pigment is 19.5 mg per gram dry cells; the acid-heated method would destroy the pigment. This study may provide some theoretic basis to further explore the natural pigment resource.