Electrophoresis of acidic glycosaminoglycans in hydrochloric acid: a micro method for sulfate determination |
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Authors: | E Wessler |
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Affiliation: | Institute of Medical Chemistry, University of Uppsala, Uppsala, Sweden |
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Abstract: | A rapid and simple method for estimation of the sulfate content of less than 1 μg of acidic glycosaminoglycans is presented. The procedure is based on the fact that the electrophoretic mobility in 0.1 M hydrochloric acid is proportional to the sulfate content of the polysaccharide. |
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