Reversal by EGTA of the enhanced secretory responsiveness of mast cells due to treatment with ouabain |
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| Authors: | T Johansen T Knudsen H Bertelsen |
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| Institution: | Department of Pharmacology, Medical School, Odense University, Denmark. |
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| Abstract: | The effect of EGTA on the enhancement by ouabain of compound 48/80-induced secretion from mast cells was compared with the effect on the Na(+)-K+ pump activity. The time-dependent secretory enhancement by ouabain was blocked by addition of EGTA to the cell suspension concomitantly with the addition of ouabain, and EGTA caused a large increase in the pump activity. Addition of 10 microM EGTA to ouabain-treated cells stopped but did not reverse the enhancement. The experiments show that the effect of ouabain was due to changes in a calcium pool utilized in compound 48/80-induced secretion following changes in the Na+,K+ pump activity. |
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