Silicone rubber membrane bioreactors for bacterial cellulose production |
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| Authors: | Masayuki Onodera Ikuro Harashima Kiyoshi Toda Tomoko Asakura |
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| Institution: | (1) Department of Applied Chemistry and Biotechnology, Niigata Institute of Technology, 1719 Fujihashi, 945-1195 Kashiwazaki, Japan;(2) Institute of Molecular and Cellular Biosciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, 113 Tokyo, Japan |
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| Abstract: | Cellulose production byAcetobacter pasteurianus was investigated in static culture using four bioreactors with silicone rubber membrane submerged in the medium. The shape
of the membrane was flat sheet, flat sack, tube and cylindrical balloon. Production rate of cellulose as well as its yield
on consumed glucose by the bacteria grown on the flat type membranes was approximately ten-fold greater than those on the
non-flat ones in spite of the same membrane thickness. The membrane reactor using flat sacks of silicone rubber membrane as
support of bacterial pellicle can supply greater ratio of surface to volume than a conventional liquid surface culture and
is promising for industrial production of bacterial cellulose in large scale. |
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| Keywords: | bacterial cellulose surface culture silicone rubber membrane Acetobacter pasteurianus |
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