Clinical proteomics: study of a cryogel |
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Authors: | Robert Daniel Barelli Stefano Crettaz David Bart Pierre-Alexandre Schifferli Jürg A Betticher Daniel Tissot Jean-Daniel |
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Institution: | Service Régional Vaudois de Transfusion Sanguine, rue du Bugnon 27, 1005 Lausanne, Switzerland. |
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Abstract: | Cryoproteins are proteins precipitating at low temperature. Usually, the precipitate contains immunoglobulins (Igs), and are therefore called cryoglobulins. Very rarely, Igs do not precipitate, but, upon cooling, form a gel. Here, we report a case of cryogel observed in a patient presenting with Waldenström's disease. Using proteomic tools, a monoclonal IgM was identified as being the cause of the gel formation. Furthermore, addition of H2O before incubation at 4°C demonstrated that the monoclonal IgM was precipitable as a type I cryoglobulin (hypocryoglobulin). |
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Keywords: | Cryoglobulin Immunoglobulin IgM 2‐DE |
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