Comparative proteomic analysis of indioside D-triggered cell death in HeLa cells |
| |
Authors: | Wong Chi Chun Wang Ying Cheng Ka-Wing Chiu Jen-Fu He Qing-Yu Chen Feng |
| |
Institution: | School of Biological Sciences, Department of Anatomy, The University of Hong Kong, Hong Kong SAR, China. |
| |
Abstract: | Medicinal plants represent a rich source of cancer drug leads. Indioside D, a furostanol glycoside isolated from Solanum mammosum, was found to possess antiproliferative activity toward a panel of human cancer cell lines. Proteomic analysis of indioside D-treated HeLa cells revealed profound protein changes related to energy production and oxidative stress, suggesting that mitochondria dysfunction plays a role in indioside D-induced apoptosis. Indioside D caused a rapid dissipation of mitochondrial transmembrane potential (DeltaPsim) and the generation of reactive oxygen species (ROS), leading to the activation of caspase-dependent apoptotic cell death. The Fas death receptor pathway was also activated following indioside D treatment, and triggered the activation of caspase-8 and cleavage of Bid, which also acted through the mitochondrial apoptosis pathway. These results suggest that indioside D induced apoptosis in HeLa cells via both intrinsic and extrinsic cell death pathways. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|