Characteristics of cellulase preparations affecting the simultaneous saccharification and fermentation of cellulose to ethanol |
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Authors: | Helen Golias Geoffrey J. Dumsday Grant A. Stanley Neville B. Pamment |
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Affiliation: | (1) Department of Chemical Engineering, University of Melbourne, Parkville, Victoria, Australia, 3052;(2) School of Life Sciences and Technology, Victoria University of Technology, Werribee Campus (W008), P.O. Box 14428, Melbourne City MC, Victoria, Australia, 8001 |
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Abstract: | The cellulase, Spezyme CP from Genencor, widely used for the simultaneous saccharification and fermentation (SSF) of cellulose to ethanol, contained substances inhibitory to the growth of Klebsiella oxytoca P2, emphasising the need to check for inhibition effects in SSF experimentation. Also, the preparation contained enough -glucosidase activity to prevent cellobiose accumulation in SSF with a conventional non-cellobiose fermenting yeast: this finding is relevant to attempts to evaluate novel recombinant cellobiose-fermenting microbial strains. |
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Keywords: | cellulase cellulose Klebsiella oxytoca P2 Saccharomyces pastorianus simultaneous saccharification and fermentation |
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