| 18种绣线菊分子身份证的构建和SCAR标记转化 |
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| 引用本文： | 刘慧民,刘一佳,吕贵娥,车代弟. 18种绣线菊分子身份证的构建和SCAR标记转化[J]. 植物生理学通讯, 2009, 0(12): 1171-1176 |
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| 作者姓名： | 刘慧民 刘一佳 吕贵娥 车代弟 |
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| 作者单位： | 东北农业大学园艺学院,哈尔滨150030 |
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| 基金项目： | 黑龙江省自然科学基金（C2007-11）和东北农业大学创新团队项目（CXZ004-3）. |
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| 摘 要： | 取北方常见的18种绣线菊,用200条RAPD随机引物进行扩增,选出10条引物扩增出的清晰稳定、重复性好的图谱进行数据分析。10条引物共检测出51个位点。对凝胶电泳得到的谱带统计结果并赋值,用IDAnalysis 1.0软件进行数据分析的结果表明,仅需6条引物对应的7个位点可将18种绣线菊完全区分。在RAPD扩增过程中,找到三裂绣线菊的特异标记SL700,并将此标记转化成SCAR标记,这一特异标记在分子水平可将三裂绣线菊与其他17种绣线菊区分开来。
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| 关 键 词： | 绣线菊 分子身份证 RAPD标记 SCAR标记 |
Establishment of Molecular ID and Conversion to SCAR Marker in Eighteen Spiraea Species |
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| LIU Hui-Min,LIU Yi-Jia,LU Gui-E,CHE Dai-Di. Establishment of Molecular ID and Conversion to SCAR Marker in Eighteen Spiraea Species[J]. Plant Physiology Communications, 2009, 0(12): 1171-1176 |
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| Authors: | LIU Hui-Min LIU Yi-Jia LU Gui-E CHE Dai-Di |
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| Affiliation: | ( College of Horticulture, Northeast Agricultural University, Harbin 150030, China) |
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| Abstract: | A total of 18 Spiraea species common in North China were amplified by 200 randomly selected primers with RAPD method. We chose 10 primers that had clear, stable and reproducible electrophoretic maps for data analysis. There were 51 polymorphic sites detected from 10 primers. The bands of gel electrophoresis were taken statistics, assigned, and analyzed by ID Analysis 1.0 software. The results showed that 18 Spiraea species could be completely distinguished by 7 sites of 6 primers. In the RAPD amplification process, a specific marker named SL700 of Spiraea trilobata was found, and SET00 was converted to SCAR marker. Using the snecific marker. Soiraea trilobata could be distinguished from other Spiraea soecies at a molecular level. |
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| Keywords: | Spiraea L. molecular ID RAPD marker SCAR marker |
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