| 亚麻胚性细胞悬浮培养体系的建立和影响因素 |
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| 引用本文： | 苗书魁,;姜丽,;计巧灵,;李文婷,;热西旦·尼格买提. 亚麻胚性细胞悬浮培养体系的建立和影响因素[J]. 植物生理学通讯, 2009, 0(12): 1181-1185 |
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| 作者姓名： | 苗书魁, 姜丽, 计巧灵, 李文婷, 热西旦·尼格买提 |
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| 作者单位： | [1]新疆大学生命科学与技术学院,乌鲁木齐830046; [2]新疆轻工职业技术学院食品工程系,乌鲁木齐830021 |
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| 基金项目： | 国家自然科学基金（30660095）和新疆教育厅重点科研项目（XJEDU2006108）. |
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| 摘 要： | 亚麻品种‘双亚五号’的胚性愈伤组织诱导最佳培养基为MB（MS无机盐加B5维生素）＋1.0mg·L^-12,4-D;细胞初始悬浮培养的最佳培养基为MB＋0.2mg·L-16-BA;细胞继代悬浮培养的最佳培养基为改良的MB（NH4NO3减半,KNO3加倍）＋0.02mg·L^-12,4-D＋0.2mg·L^-16-BA;适宜的蔗糖量为50g·L^-1;适宜的继代接种量为1.0～1.5g·（25mL）^-1;继代间隔为5～7d。
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| 关 键 词： | 亚麻 胚性愈伤组织 悬浮细胞系 |
Establishment and Influence Factors of Embryogenic Cell Suspension Lines of Linum usitatissimum L. in vitro |
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| Affiliation: | MIAO Shu-Kui, JIANG Li, JI Qiao-Ling, LI Wen-Ting, Rexidan·Nigemaiti ( 1College of Life Science and Technology, Xinjiang University, Urumqi 830046, China; 2Food Engineering Department, Xinjiang Institute of Light Industry Technology, Urumqi 830021, China) |
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| Abstract: | The optimum induction medium for the embryogenic callus of Linum usitatissimum ＇Shuangya No. 5＇ was MB （MS inorganic salts supplemented with B5 vitamins）＋ 1.0 mg·L^-1 2,4-D. The optimum medium for initial culture of suspension cells was liquid MB supplemented with 0.2 mg·L^-1 6-BA. The suspension cells were cultured on modified MB liquid medium （NH4NO3 halved, KNO3 doubled） supplemented with 0.02 mg·L^-1 2,4- D＋0.2 mg·L^-16-BA for subculturing. The suitable sucrose concentration was 50 g·L^-1. The optimal inoculative densities were 1.0-1.5 g·（25 mL）^-1, and the subculture period was 5-7 d. |
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| Keywords: | Linum usitatissimum embryogenic callus cell suspension lines |
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