Evolution of Nitrogen Oxide(s) during In Vivo Nitrate Reductase Assay of Soybean Leaves |
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| Authors: | Harper J E |
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| Affiliation: | United States Department of Agriculture, University of Illinois, Urbana, Illinois 61801. |
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| Abstract: | Studies were conducted to quantitate the evolution of nitrogen oxides (NO(x)) from soybean [Glycine max (L.) Merr.] leaves during in vivo nitrate reductase (NR) assays with aerobic and anaerobic gas purging. Anaerobic gas purging (N2 and argon) consistently resulted in greater NO(x) evolution than did aerobic gas purging (air and O2). The evolution of NO(x) was dependent on gas flow rate and on NO2− formation in the assay medium; although a threshold level of NO2− appeared to exist beyond which the rate of NO(x) evolution did not increase further. |
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