| 大肠杆菌热激反应研究及其在重组蛋白表达中的应用 |
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| 引用本文： | 乐易林. 大肠杆菌热激反应研究及其在重组蛋白表达中的应用[J]. 微生物学通报, 2013, 40(11): 2090-2096 |
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| 作者姓名： | 乐易林 |
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| 作者单位： | 江苏大学 环境与安全工程学院 生物质能源研究所 江苏 镇江 212013 |
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| 基金项目： | 江苏省高校自然科学研究项目(No. 12KJB180002); 镇江市农业科技支撑计划项目(No. NY2012032); 江苏大学高级专业人才科研启动基金项目(No. 10JDG117) |
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| 摘 要： | 当大肠杆菌所处的环境温度忽然升高时, 细胞体内会激发热激反应, 体内会迅速合成多种热激蛋白, 由热激转录因子调控的热激蛋白主要包括一些分子伴侣、蛋白降解酶、折叠辅助蛋白等。热激蛋白可以促进蛋白正确折叠, 降解错误折叠的蛋白。主要介绍大肠杆菌热激蛋白的表达调控及其功能, 利用热激转录因子发展的新型温控分泌表达系统及其在蛋白可溶性表达中的应用, 以及热激分子伴侣与重组蛋白共表达的研究进展。
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| 关 键 词： | 大肠杆菌 表达系统 热激反应 pHsh载体 热激诱导 |
Heat shock response in Escherichia coli: research progress and its application on recombinant protein expression systems |
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| LE Yi-Lin. Heat shock response in Escherichia coli: research progress and its application on recombinant protein expression systems[J]. Microbiology China, 2013, 40(11): 2090-2096 |
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| Authors: | LE Yi-Lin |
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| Affiliation: | Biofuels Institute, School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang, Jiangsu 212013, China |
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| Abstract: | The heat shock response (HSR) in Escherichia coli is de?ned as the cellular response to temperature increase. A set of proteins are synthesized when E. coli is stressed with elevated temperatures. These proteins are known as heat shock proteins (Hsp) many of which are molecular chaperones, proteases and folding factors. Some of heat shock proteins can promote proper folding of proteins and the degradation of misfolded proteins. This paper introduces the characterization and regulation of the heat shock proteins, the application of heat inducible secretion vector pHsh-ex to recombinant protein expression, and the resent research progress on chaperone co-expression systems. |
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| Keywords: | E. coli expression system heat shock response pHsh vector heat shock induction |
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