Synthesis and assembly of microtubule proteins in Tetrahymena pyriformis |
| |
| Authors: | S Tamura |
| |
| Affiliation: | 1. Interventional Regenerative Medicine and Imaging Laboratory, Stanford University School of Medicine, Department of Radiology, Palo Alto, CA 94304, USA;1. Key Laboratory of Pu-er Tea Science, Ministry of Education, Yunnan Agricultural University, Kunming, 650201, China;2. College of Science, Yunnan Agricultural University, Kunming, 650201, China;3. College of Food Science and Technology, Yunnan Agricultural University, Kunming, 650201, China;4. State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Kunming, 650201, China;1. Andalusian Center for Developmental Biology (CABD), CSIC – UPO – JA, Seville, Spain |
| |
| Abstract: | - 1.1. Amounts of cilia-type microtubule proteins in a cell and a macronucleus were estimated to be about 7% and 0.2% of those of the total protein within the cell respectively, at various stages of the cell. These values imply that various microtubules in the cell include a common protein(s).
- 2.2. About 70% of newly synthesized microtubule proteins were transferred to insoluble fraction within 3 min. This rapid transfer was proved to occur by the assembly of newly synthesized protein into insoluble structures, from the experimental results using cycloheximide and colchicine.
- 3.3. The synthetic activity of microtubule proteins and the rate of assembly of the proteins into higher structures (insoluble fration) were shown to be nearly constant through the stages after the end of the heat-treatment up to synchronous division.
|
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
