Potentiation of glutamate-activated currents in isolated hippocampal neurons |
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Authors: | Y I Zilberter V V Uteshev S N Sokolova L G Motin H H Eremjan |
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Affiliation: | Institute of General Pathology and Pathological Physiology, Moscow, Union of Soviet Socialist Republics. |
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Abstract: | "Fast chemical stimulation" was shown to induce potentiation of glutamate-activated currents in neurons isolated from rat hippocampus. A fast application system allowed solution changes up to a rate of 20 Hz. In Mg2(+)-free solution, the response to glutamate application immediately after repetitive stimulation with glutamate plus glycine was increased by 25%-88%, returning to control levels over 10-15 min. Enhancement of glutamate-induced currents was also seen after stimulation with solutions containing aspartate or NMDA plus glycine. Aspartate-induced currents were not potentiated. These and other observations demonstrate that in a purely "postsynaptic" system, short-term potentiation can be induced and is mediated via NMDA receptors whereas the potentiated current is carried via non-NMDA glutamate receptor channels. |
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