Over-expression and production of plant allergens by molecular farming strategies |
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Authors: | Obermeyer Gerhard Gehwolf Renate Sebesta Wolfgang Hamilton Nichola Gadermaier Gabriele Ferreira Fatima Commandeur Uli Fischer Rainer Bentrup Friedrich-Wilhelm |
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Affiliation: | Institute of Plant Physiology, University of Salzburg, Hellbrunnerstr. 34, 5020 Salzburg, Austria. gerhard.obermeyer@sbg.ac.at |
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Abstract: | Recombinant allergens have become a valuable tool for diagnosis and may also be used for therapy in the near future. To supply the required large amounts of functional recombinant proteins on a cost-effective basis, the production of allergens in plants by molecular farming is an alternative to microbial expression systems. Especially as post-translational modifications of the allergens, e.g., phosphorylation and glycosylation, may be important for recognition by the human immune system, the plant-based production of recombinant allergens enables the correct folding, glycosylation, and other modifications of the recombinant allergen. An introduction to the methods for plant transformation via the tumor-inducing bacterium, Agrobacterium tumefaciens, is given in this paper. |
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