Reduction of sialic acid O-acetylation in human colonic mucins in the adenoma-carcinoma sequence |
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| Authors: | Anthony P. Corfield Neil Myerscough Bryan F. Warren Paul Durdey Christos Paraskeva Roland Schauer |
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| Affiliation: | (1) University Department of Medicine, Histopathology, Bristol Royal Infirmary, Bristol, BS2 8HW, UK;(2) University Department of Surgery, Bristol Royal Infirmary, Bristol, BS2 8HW, UK;(3) University Department of Pathology and Microbiology, School of Medical Sciences, Bristol, BS2 8TD, UK;(4) Biochemisches Institut, Christian Albrechts Universitat., Olshausenstrasse 40, D-24098 Kiel, Germany |
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| Abstract: | The oligo-O-acetylation of sialic acids found in normal colonic mucins is greatly reduced in colorectal cancer. Mucins prepared from cancer tissue in adenocarcinoma showed this reduction, while normal O-acetylation was detected in resection margin and control cases and total mucin sialic acid content was significantly decreased in cancer vs control samples. A reduction of the O-acetyl transferase activity catalysing the O-acetylation reaction was also found. A series of cultured human colorectal cell lines derived from the same premalignant adenomatous line, and representative of the adenoma-carcinoma sequence were examined and revealed a depletion of oligo-O-acetylation in the original diploid premalignant line, re-expression in a further premalignant line and reduction in malignant mucinous and adenocarcinoma cell lines. Reduction of sialic acid O-acetylation appears as an early event in the process of malignant transformation in human colorectal cancer. |
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| Keywords: | sialic acids O-acetylation sialate O-acetyltransferase colon cancer mucin Bovine submandibular gland mucus glycoprotein (BSM) horse radish peroxidase (HRP) abbreviations for sialic acids Neu5Ac, Neu5Gc, Neu9Ac5Gc, Neu5,9Ac2, Neu5,8,9Ac3, Neu5,7,8,9Ac4 as before [1] |
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