| HIV gp41基因分段低温诱导表达 |
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| 引用本文： | 袁玉华,毕昌昊,李菊,王学谦,耿运琪,陈启民. HIV gp41基因分段低温诱导表达[J]. Virologica Sinica, 2004, 19(2): 179-181 |
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| 作者姓名： | 袁玉华 毕昌昊 李菊 王学谦 耿运琪 陈启民 |
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| 作者单位： | 南开大学生命科学学院,天津300071 |
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| 基金项目： | 高等学校博士学科点专项科研基金(2000005512) |
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| 摘 要： | Clone N3 and C from Human immunodeficiency virus(HIV) gp41 gene were expressed using the pET expression system. When induced by IPTG at 37℃, both two clones did not express in E.coli BL21(DE)3. Howerver, when induced at 16℃, the two clones were both overexpressed, and the amount of the product was about 20% of the total bacteria protein. In Western blotting test, the protein product could react with HIV-positive serum. After IPTG induction, E. coli cells had much higher death rate at 37℃ than at 16℃; [^3H]uridine release assay also showed that after IPTG induction, E. coli had a higher release at 37℃. The results suggested that overexpression of the two proteins was due to their decreased toxicity at lower temperature.
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| 关 键 词： | 人类免疫缺陷病毒 HIV GP41跨膜蛋白 基因 低温诱导 表达 毒性 |
Low-temperature Induced Expression of HIV gp41 Gene Segments |
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| YUAN Yu-hua,BI Chang-hao,LI Ju,WANG Xue-qian,GENG Yun-qi,CHENG Qi-min. Low-temperature Induced Expression of HIV gp41 Gene Segments[J]. 中国病毒学(英文版), 2004, 19(2): 179-181 |
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| Authors: | YUAN Yu-hua BI Chang-hao LI Ju WANG Xue-qian GENG Yun-qi CHENG Qi-min |
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| Abstract: | |
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| Keywords: | Human immunodeficiency virus (HIV) gp41 gene Low temperature induction Toxic |
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