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DNA methylation in the Alcohol dehydrogenase-1 gene of maize
Authors:Virginia Walbot  Christine Warren
Institution:(1) Department of Biological Sciences, Stanford University, 94305-5020 Stanford, CA, USA
Abstract:Using a battery of methylation-sensitive restriction enzymes, cytosine methylation at 23 sites in a 7.6 kb region surrounding the Alcohol dehydrogenase-1 (Adh1) gene was measured in DNA prepared from immature maize cobs. Both the 5prime upstream region and the entire coding region were hypomethylated in the two alleles examined. Methylation in Adh1 is independent of changes in Mutator transposable element methylation. The role of DNA methylation in Adh1 gene regulation is discussed.
Keywords:Alcohol dehydrogenase  DNA methylation  Zea mays
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