Persistent expression of the ATP-binding cassette transporter, Abcg2, identifies cardiac SP cells in the developing and adult heart |
| |
Authors: | Martin Cindy M Meeson Annette P Robertson Scott M Hawke Thomas J Richardson James A Bates Susan Goetsch Sean C Gallardo Teresa D Garry Daniel J |
| |
Affiliation: | Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, TX 75390-8573, USA. |
| |
Abstract: | Stem cells are important in the maintenance and repair of adult tissues. A population of cells, termed side population (SP) cells, has stem cell characteristics as they have been shown to contribute to diverse lineages. In this study, we confirm that Abcg2 is a determinant of the SP cell phenotype. Therefore, we examined Abcg2 expression during murine embryogenesis and observed robust expression in the blood islands of the E8.5 yolk sac and in developing tissues including the heart. During the latter stages of embryogenesis, Abcg2 identifies a rare cell population in the developing organs. We further establish that the adult heart contains an Abcg2 expressing SP cell population and these progenitor cells are capable of proliferation and differentiation. We define the molecular signature of cardiac SP cells and compare it to embryonic stem cells and adult cardiomyocytes using emerging technologies. We propose that the cardiac SP cell population functions as a progenitor cell population for the development, maintenance, and repair of the heart. |
| |
Keywords: | ATP-binding cassette transporter Abcg2 Cardiac SP cells |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|