Partial characterisation of and the effect of insect growth hormones on the ribosomes and polyribosomes of the nematode, Panagrellus redivivus |
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Authors: | R.D.W. Dennis |
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Affiliation: | Department of Plant Pathology, Waite Agricultural Research Institute, University of Adelaide, Glen Osmond, South Australia 5064 Australia |
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Abstract: | An investigation of some of the properties of the ribosomes and polyribosomes of Panagrellus redivivus revealed that: the polyribosomal RNA was resolvable into eight species, four of which possessed typical S-values and M.W.s and closely resembled those of Aphelenchus avenae; the estimated S-value of the ribosomes was 92; the polyribosomes were mainly free and not membrane-bound: and, the polyribosomes showed a low level of activity in in vitro amino-acid incorporation. The polysomes (double-labelled or unlabelled) revealed no effect of synthetic juvenile hormone or ß-ecdysone (1 × 10?5M) on their polyribosomal profile, at intervals up to and including 19 h of incubation. |
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Keywords: | Nematode ribosomes polyribosomes double-labelled polyribosomes insect growth hormones total nucleic acids |
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