The in vivo effects of rafoxanide on the energy metabolism of Fasciola hepatica |
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Authors: | RA Cornish CA Behm RW Butler C Bryant |
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Institution: | 1. Department of Zoology, Australian National University, A.C.T., Australia;2. Merck, Sharpe and Dohme (Australia) Pty. Ltd., Ingleburn, N.S.W., Australia |
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Abstract: | Adult F. hepatica were obtained from sheep which had received a single dose of rafoxanide at the therapeutic dose rate (7·5 mg/kg body weight). Flukes were recovered 12 and 24 h after the sheep were treated. No flukes were present after 4 days. Plasma levels of the drug were high after 24 h and remained so at 4 days. Flukes were being expelled from the liver 24 h after treatment. Glycogen levels within the flukes were diminished in the 24 h treated group, as were concentrations of ATP. These effects were not apparent in the 12 h treated group. Fluctuations in glucose, G6P, F6P and pyruvate pools were observed in both groups. The effects of rafoxanide were irreversible after 24 h exposure to the drug. Flukes from the treated sheep were incubated for 6 and 24 h in a simple maintenance medium with added glucose. They showed progressive deterioration in energy status. The results are considered in the context of the mode of action of rafoxanide. |
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Keywords: | anthelmintics rafoxanide energy metabolism ATP ADP AMP adenosine tri- di- and monophosphate F6P fructose-6-phosphate G6P glucose-6-phosphate |
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