Enzymatic saccharification of pretreated corn stover in a fed-batch membrane bioreactor |
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Authors: | Mingjia Zhang Rongxin Su Qiang Li Wei Qi Zhimin He |
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Institution: | 1. State Key Laboratory of Chemical Engineering, Tianjin Key Laboratory of Membrane Science and Desalination Technology, School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, People??s Republic of China
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Abstract: | Enzymatic hydrolysis of corn stover was performed in an integrated membrane bioreactor (MBR) incorporating a 10 kDa flat sheet polysulfone membrane to increase cellulose conversion and to reduce enzyme dosage. Several pretreatment methods and semi-continuous MBR were examined to investigate their effect on the glucose yield and enzyme utilization efficiency. Compared with conventional batch reactor (CBR), cellulose conversion increased by 5% in a MBR because of the removal of glucose and cellobiose inhibitors. More than 15% increment in cellulose conversion was obtained using fed-MBR, and the reaction rate improved significantly. Enzyme utilization efficiency in a fed-batch MBR were 1.94-fold of CBR and 1.34-fold of fed-CBR for corn stover pretreated by soaking in aqueous ammonia and 3.31-fold of CBR and 1.32-fold of fed-CBR for corn stover pretreated by diluted sulfuric acid?Csodium hydroxide. |
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