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Equilibrium protein folding-unfolding process involving multiple intermediates |
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| Authors: | Hung Hui-Chih Chen Yu-Hou Liu Guang-Yaw Lee Hwei-Jen Chang Gu-Gang |
| Institution: | (1) Department of Life Science, National Chung-Hsing University, Taichung, Taiwan;(2) Institutes of Life Science and Biochemistry, National Defense Medical Center, Taipei, Taiwan;(3) Institute of Immunology, Chung-Shan Medical University, Taichung, Taiwan;(4) Department of Life Science, National Yang-Ming University, Shih-Pai, 112 Taipei, Taiwan |
| Abstract: | Mathematical models for the protein folding-unfolding process involving multiple intermediates have been derived. Computer fitting of the experimental data to this model generates various thermodynamic parameters for the folding-unfolding process. In this way, the complex folding-unfolding process of the multi-domain proteins can be analysed in a quantitative way. The application of the folding-unfolding model involving seven stages in human placental alkaline phosphatase is described. These authors contributed equally to this paper. |
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