Biotransformation of albendazole by fungi |
| |
Authors: | G S Prasad S Girisham S M Reddy K Srisailam |
| |
Institution: | (1) Department of Microbiology, Kakatiya University, Warangal, AP, India;(2) Department of Pharmacy, Kakatiya University, Warangal, AP, India;(3) Vaagdevi College of Pharmacy, Ramnagar, Hanamkonda, Warangal, 506001, AP, India |
| |
Abstract: | Twenty-five fungal cultures were screened for their ability to transform the anthelmintic drug albendazole. A filamentous
fungi Cunninghamella blakesleeana transformed albendazole to three metabolites in significant quantities. The transformation of albendazole was identified
by HPLC. Based on the LC-MS-MS data, two metabolites were predicted to be albendazole sulfoxide and albendazole sulfone, the
major mammalian metabolites reported previously. A new N-methylated metabolite of albendazole sulfoxide was also produced, where the methylation took place on the N-atom of the imidazole
ring system. A temperature of 30°C, pH of 8 and high substrate concentrations produced highest transformation of albendazole.
Among the various concentrations studied, 2% w/v of glucose produced highest transformation. The results reveal that the microbial
model can be used to produce large quantities of mammalian metabolites. |
| |
Keywords: | Albendazole Albendazole sulfone Albendazole sulfoxide Biotransformation Cunninghamella blakesleeana Metabolites Microorganisms |
本文献已被 SpringerLink 等数据库收录! |
|