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猪源肠道乳酸菌的分离与生物学特性研究
引用本文:杨霞,陈陆,王新卫,张红渠,常洪涛,刘红英,王川庆.猪源肠道乳酸菌的分离与生物学特性研究[J].中国微生态学杂志,2010,22(5):393-397.
作者姓名:杨霞  陈陆  王新卫  张红渠  常洪涛  刘红英  王川庆
作者单位:河南农业大学牧医工程学院,河南,郑州,450002
摘    要:目的分离猪消化道中的乳酸菌。方法无菌采集健康猪的新鲜粪便,接种MRS培养基,厌氧培养分离乳酸菌。并对分离菌形态学,生化特性,产酸性,耐酸性,耐胆汁性,高温耐受性,抑菌性等方面进行研究。结果分离到5株嗜酸乳杆菌,分离的细菌对2株致病菌明显具有抑制性。结论为研制高效专一的猪用微生态制剂奠定基础。

关 键 词:  乳酸杆菌  分离鉴定  生物学特性

Study on isolation and biological characteristics of Lactobacillus in intestines of pig
YANG Xia,CHEN Lu,WANG Xin-wei,ZHANG Hong-qu,CHANG Hong-tao,LIU Hong-ying,WANG Chuan-qing.Study on isolation and biological characteristics of Lactobacillus in intestines of pig[J].Chinese Journal of Microecology,2010,22(5):393-397.
Authors:YANG Xia  CHEN Lu  WANG Xin-wei  ZHANG Hong-qu  CHANG Hong-tao  LIU Hong-ying  WANG Chuan-qing
Institution:YANG Xia,CHEN Lu,WANG Xin-wei,ZHANG Hong-qu,CHANG Hong-tao,LIU Hong-ying,WANG Chuan-qing(Engineering College of Animal Husbandry and Veterinary Science,Henan Agricultural University,Zhengzhou 450002,China)
Abstract:Objective To separate lactic acid bacteria from pig digestive tract.Method The fresh stool samples were collected from healthy piglets and inoculated in MRS culture medium to isolate lactobacillus by using anaerobic culture.The isolates were investigated according to biological characteristic tests such as their morphology,biochemical characters,lactic acid production,acid resistance,tolerance to bile salts,high temperature tolerance and antagonistic property.Result Five strains of Lactobacillus acidophilus...
Keywords:Pig  Lactobacillus  Identification  Biology characteristic  
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