| 响应面法优化叶酸高产菌发酵培养基及发酵条件 |
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| 引用本文： | 刘艳,胡刘秀,叶生梅,阚清华.响应面法优化叶酸高产菌发酵培养基及发酵条件[J].生物数学学报,2011(3):533-539. |
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| 作者姓名： | 刘艳 胡刘秀 叶生梅 阚清华 |
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| 作者单位： | 安徽工程大学生化工程学院微生物发酵安徽省工程技术研究中心;芜湖张恒春药业有限公司; |
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| 基金项目： | 安徽工程科技学院重点青年基金项目(2007YQ006zd); 芜湖市科技计划重点项目(2008507) |
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| 摘 要： | 采用响应面法对叶酸产生菌产朊假丝酵母Y2.12的发酵发酵培养基及发酵条件进行优化.首先,采用Plackett-Burman方法对影响发酵各因素的效应进行评价,筛选出有显著效应的3个因素：碳源乳糖、pH值和摇床转速;并通过Box-Benhnken设计和响应面分析法对这3个主要影响因素进行分析.结果表明,优化后这3个因素的最佳值为摇床转速196r/min、pH7和碳源乳糖含量为6.25%.采用优化后的发酵培养基及发酵条件进行摇瓶发酵,叶酸的含量可达23.14±0.13mg/L,取得了很好的优化效果.
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| 关 键 词： | 响应面设计法 叶酸 发酵条件 |
Optimization of Culture Medium and Fermentation Conditions for Highly Folic Acid Production by Response Surface Methodology |
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| LIU Yan HU Liu-Xiu YE Sheng-Mei KAN Qing-Hua.Optimization of Culture Medium and Fermentation Conditions for Highly Folic Acid Production by Response Surface Methodology[J].Journal of Biomathematics,2011(3):533-539. |
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| Authors: | LIU Yan HU Liu-Xiu YE Sheng-Mei KAN Qing-Hua |
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| Institution: | LIU Yan~1 HU Liu-Xiu~2 YE Sheng-Mei~1 KAN Qing-Hua~1 (1 College of Biochemistry Engineering,Anhui Polytechnic University,Microorganism Fermentation Engineering and Technology Research Center of Anhui Province,Wuhu Anhui 241000 China) (2 Wu Hu Zhang Heng Chun Medicine CO.,LTD,Wuhu Anhui,241009 China) |
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| Abstract: | The response surface methodology was used to optimize the culture medium and fermentation conditions of Candida utili Y2.12.In the first step of optimization,carbon source-corn flour,pH and the speed of shaker and were found to be the important factors by Plackett-Burman design.Then,the optimal value of three important factors were determined by Box-Benhnken design and response surface methodology.The result was the speed of shaker 196 r/min,pH 7 and 6.25%corn flour.After the optimization,the yield of folic... |
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| Keywords: | Response Surface Methodology Folic acid Fermentation condition |
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